Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 68)

• Econo-Pac High S Low Pressure Chromatography Cartridges: These cartridges are available in
1 ml and 5 ml formats to accommodate most sample loads. They are recommended for method
scouting and for first step purification of crude samples. They are based on 50 µm Macro-Prep
®
supports. Refer to bulletin 1985.
• Macro-Prep ® High S Support: This is a strong cation exchanger containing sulfonic acid functional
groups with a 50 µm particle size. It is ideal for purification of basic and neutral proteins and
peptides. Refer to bulletins 1840 (A-200), 1917, and 2079.
2.10.3 Anion Exchange: DEAE Weak Anion Exchange
The DEAE weak anion exchanger chemistry is available in the following formats:
• Bio-Scale DEAE Prepacked Medium Pressure Columns: These columns are designed for high
resolution separations of proteins, peptides, and polynucleotides in analytical to semipreparative
medium pressure applications. They are available in four column sizes. Methods developed on the
Bio-Scale columns can be transferred to production scale using the Macro-Prep
® 10 µm supports.
Refer to bulletins 1930, 1946, and 2079.
• Econo-Pac DEAE Blue Low Pressure Chromatography Cartridges: These cartridges are
available in 1 ml and 5 ml formats to accommodate most sample loads. They are recommended for
method scouting and for first step purification of crude samples. They are based on 50 µm Macro-
Prep
® supports. Refer to bulletin 1946.
• Macro-Prep ® DEAE Support: This is a weak anion exchanger containing diethylaminoethyl
functional groups with a 50 µm particle size. It is ideal for purification of acidic and neutral proteins
and peptides. Refer to bulletin 1840 (A-400).
2.10.4 Cation Exchange: Carboxy Methyl (CM) Weak Cation Exchange
The CM weak cation exchanger chemistry is available in the following formats:
• Econo-Pac CM Low Pressure Chromatography Cartridges: These cartridges are available in 1
ml and 5 ml formats to accommodate most sample loads. They are recommended for method
scouting and for first step purification of crude samples. They are based on 50 µm Macro-Prep
®
supports.
2.10.5 Ceramic Hydroxyapatite (CHT)
The CHT chemistry is available in the following formats:
• Bio-Scale CHT Type I Prepacked Medium Pressure Columns: These columns are designed for
high resolution separations of proteins, peptides, and polynucleotides in analytical to
semipreparative medium pressure applications. They are available in four column sizes. Methods
developed on the Bio-Scale columns can be transferred to production scale using the Macro-Prep
®
10 µm supports. Refer to bulletins 1929, 1946, 2079, and 2156.
• Econo-Pac CHT-II Low Pressure Chromatography Cartridges: These cartridges are available in
1 ml and 5 ml formats to accommodate most sample loads. They are recommended for method
scouting and for first step purification of crude samples. They are based on 20 µm Macro-Prep
®
supports. Refer to bulletin 1946.
DESCRIPTION OF SYSTEM COMPONENTS SYSTEM OVERVIEW
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