Modes of operation system operation 7-30 – Bio-Rad BioLogic Signal Import Module (SIM) User Manual

Page 159

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7.4

RUN SCREEN

The Run screen displays a run in progress. All data associated with the run are automatically saved. An
example of a run in progress is shown in Figure 7-5 a and b, below. Table 7-13 discusses the buttons which
may be used to control the run. In addition, if the EZLogic Integration software is installed, the Integ. toolbar
button replaces the Log button.

Figure 7-5a. Run Screen showing a Run in Progress with a Volume-based Chromatogram

and "Rack and Tube#" fraction numbering

Advance

Frac. Collector

Divert Valve

100.0% Buffer B

50.0

UV Detector

Set

UV Range

Zero

Baseline

Event
mark

Cond Range

500

Protocol: > 1 0:00.0 Collection Fractions of size 2.00 ml during entire run

0.00

1

A

2

3

4

5

6

7

8

9

10

11

12

13 14

10.00

20.00

5.00

15.00

25.00

AU

mS/cm

Collect

Waste

Grad. Pump

Chart Recorder

1000

High psi

0

Low psi

QuadTec

Zero

Baseline

0.01

0.0

50.0

40.0

30.0

20.0

10.0

-10.0

Conductivity

UV

Pause

Abort

Hold Full View

PULL DOWN MENU
TO SELECT LEFT TRACE

PULL DOWN MENU

TO SELECT RIGHT TRACE

ACTIVE

ACTIVE

0.100

0.075

0.050

0.025

-0.000

BioLogic Duo-Flow - <user name> - <project name> - <method name> - <run name>

File

View

Utilities

Options

Edit

Method

New

Method

New

Run Browser

Manual Setup Protocol

wash

load

1

2

Run

Notes

Report

PostRun Log

Window

Help

Settings

WL1 - 280nm

0.40

WL2 - 260nm

0.15

WL3 - 214nm

2.00

WL4 - 405nm

0.00

Flow Rate

EGP %B

0 %B

0.00 ml/min

0%

% Split

Run Time

0:00.0

Run Volume

0.0 ml

Step Time Left

0:00.0

Fraction Vol. Left

0.0 ml

WL1 - 280nm

Port 2: User Valve; Position 1

1.00ml/min

0 %B2

Maximizer + Gradient Pump: F10

UV

Conductivity

438 psi

1.003 AU

1.23 mS/cm

QuadTec

Econo Gradient

Pump

SIM1/pH

SIM1/SIG

0.548 Volt

7.00 pH

Edit

Bio-Rad

Web

Milliliters

Fractions

Rack Pos:
Tube #:

B

A

Scout: Method 7 - Scout Run - [S] Scout Buffer A A1 [ 1 of 1 ]

> [S] Scout Buffer A A1

MODES OF OPERATION

SYSTEM OPERATION

7-30

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