Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 131)

7.1 MANUAL SCREEN
The Manual screen is used to control and monitor the operation of the DuoFlow Workstation and each of its
peripheral devices. This interface is divided into three basic groups of dialogs and includes control panels, a
real time chromatogram display and a status bar.
Figure 7-2. Manual Screen, for the BioLogic DuoFlow system connected to a Maximizer, BioFrac
Fraction collector, QuadTec Detector, Econo Gradient Pump, and Four Valves
From the Manual screen you can control the following:
Gradient Pump: This panel is used to control buffer composition, flow rate and pressure limits (see
Table 2-3) as well as to start and stop the pumps. The Start/Stop buttons are used to start and stop the
pumps. The Set button is used to make changes to the composition, flow rate or pressure limits while the
pumps are running.
BioLogic Duo-Flow - <user name> - <project name> - <method name> - <run name>
Edit
Method
New
Method
New
Run Browser Manual Setup Setup Protocol
wash
load
1
2
Run Notes Report PostRun Log
Advance
Fraction Collector: BioFrac
START
Maximizer+Gradient Pump: F10
pH
1.00
100
0 Inlet B
Flowrate ml/min
%
%
High
limit
0
1000
Low
limit
psi.
AVR7-3 at port 4
Workstation Valves
SVT3-2 at port 1 SV5-4 at port 2
P L 2 1
2 1
3 4
STOP START STOP START STOP
Set
Set
psi.
Econo Gradient Pump 1
EGP %B
1.000
0
0.00 % Split
Flow
Direction
Flowrate ml/min
%
%
Set
Tube #:
Rack Pos:
Tube #:
A
Tube #: Grid #:
ml
Vol. left:
Mode: System Local Mode: System Local Mode: System Local
Start:
Rack:
End:
Fraction size:
1 1F
1A
1
Lamp Type
Range
Deuterium
190 - 370 nm
90
1.00
nm 280
A
B
I
AVR9-8 at port 5
3 7
8 2
6
5
4
I
QuadTec Detector
ON OFF
Zero Baseline
Wavelength Selection
2.00
100% Buffer B
0 2 4 6 8 10
Min. Tenth AU mS/cm
1.50
1.00
0.50
50
0.00
500.0
0.0
400.0
300.0
200.0
100.0
Fractions
UV Conductivity
Clear
Traces
Resize
WL1 - 280nm
0.0041 AU
WL2 - 260nm
-0.00001 AU
WL3 - 214nm
0.00021 AU
WL4 - 405nm
0.00004 AU
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
% Split
1.00ml/min 0 %B2
Maximizer + Gradient Pump: F10 UV Conductivity
438 psi 1.003 AU 1.23 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH SIM1/SIG
0.548 Volt 7.00 pH
Settings
nm 260
nm 214
nm 405
CONTENTS
MAY VARY
DEPENDING ON
CONFIGURATION
File View Utilities Options Window Help
Bio-Rad
Web
Rack: F1 (12-13 mm tubes)
Setup
Mode: System Local
B
MODES OF OPERATION SYSTEM OPERATION
7-2