Bio-Rad BioLogic Signal Import Module (SIM) - Manual (Page 141)

Table 7-4.
Load/Inject Sample
The Edit Load/Inject Sample dialog is used to program sample injection. The appearance of this dialog and
its associated tabs depend on the injection type selected and the devices configured in the device setup.
• Injection Buffers: Displays all the available buffers. The names displayed for Buffer A and Buffer B
are the names assigned to the pump inlets or inlet valves in the device setup (see Chapter 7.2.2).
• Composition: Allows you to set the buffer composition used to push the sample onto the column
(e.g., 75% A and 25% B).
When Buffer Blending is defined in the device setup the Buffer System, pH and %B fields are displayed.
• pH: Allows you to enter the pH for the current step. Displayed when Buffer Blending is included in
the device setup.
• %B: Allows you to set the buffer composition for the step as a percentage of the 1x salt
concentration. Displayed when Buffer Blending is included in the device setup.
Load/Inject
Sample
Edit Load/Inject Sample
Step 3, Volume = 1.00 ml
Load/Inject Sample
Fill Before Inject
Buffer A1
OK Cancel
Rinse After Inject
100 A
Buffer B1
Injection Buffers
Type
% Comp.
0
1.0
Volume (ml.)
1.00 B
Flow (ml./min)
Static Loop
Dynamic Loop
Direct Inject
Edit Load/Inject Sample
Step 2, Volume = 1.00 ml
Load/Inject Sample Fill Before Inject
OK Cancel
Rinse After Inject
Type
Static Loop
Dynamic Loop
Direct Inject
Auto Inject Valve will move to INJECT at start of step and return to LOAD at end of step.
Buffer System: Tris (25 mM)
pH Range = 7.20 to 9.20
Solution A1: Tris.HCI (50 mM)
Solution A2: Tris (50 mM)
Solution B1: Degassed Water
Solution B2: Sodium Chloride (2.00M)
Molarity at 100%B = 1.0 M
1.0
Volume (ml.)
1.00
Flow (ml./min)
7.00
pH
0
%B
Auto Inject Valve will move to INJECT at start of step and return to LOAD at end of step.
Load/Inject Sample Screen
Load/Inject Sample Screen
with a Maximizer in Use
MODES OF OPERATION SYSTEM OPERATION
7-12