Modes of operation system operation 7-38 – Bio-Rad BioLogic Signal Import Module (SIM) User Manual

Page 167

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7.5

POST RUN Screen

The Post Run screen enables peak annotation (“tagging”) and data export. To enter the Post Run screen,
open a run file in the Browser screen, or when a run is finished choose PostRun from the toolbar. Two
chromatograms are viewed: the main chromatogram in the lower large window and the reference
chromatogram in the upper right window. In the upper reference chromatogram, use the vertical scrollbar to
adjust the scale.

Figure 7-11a. Post Run Screen showing a Volume-based Chromatogram

and "Rack and Tube#" fraction numbering

Tags

AU

SA

FullView Print

Del Tag

Activity

Edit

View

Utilities

Options

Edit

Method

New

Method

New

Run

Manual

Setup

Values at Cursor

Current Tagging Trace:

QuadTec-3 (214 nm)

Run Time:

Gradient:

QuadTec-1 (280 nm):
QuadTec-2 (260 nm):
QuadTec-3 (214 nm):
QuadTec-4 (405 nm):

Conductivity:

GP pressure:

No Trace:

00:03:38.4
21% Buffer B
0.0345 AU
0.0228 AU
0.4762 AU
0.0021 AU
8.47 mS/cm
375.53 psi

Protocol

wash

load

1

2

Run

Notes

Report

PostRun

Settings

Log

Help

File

Browser

50

100% Buffer B

QuadTec-3 (214 nm)

AU

GP pressure

50.0

0.0

-10.0

40.0

30.0

20.0

10.0

Fractions

BioLogic

Duo-Flow

- <user name> - <project name> - <method name> - <run name>

WL1 - 280nm

0.40

WL2 - 260nm

0.15

WL3 - 214nm

2.00

WL4 - 405nm

0.00

Flow Rate

EGP %B

0 %B

0.00 ml/min

0%

Split

1.00ml/min

0 %B

Gradient Pump: F-40

UV

Conductivity

438 psi

0.03775 AU

359 mS/cm

QuadTec

Econo Gradient

Pump

SIM1/pH

SIM1/SIG

0.953 Volt

0.00 pH

A

mS/cm

B

0.00

1

1

A

2

3

4

5

6

7

8

9

10

11

12

13 14

10.00

20.00

5.00

15.00

25.00

2

3

4

0.100

0.075

0.050

0.025

-0.000

Milliliters

Rack Pos:
Tube #:

MODES OF OPERATION

SYSTEM OPERATION

7-38

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