Leica Biosystems PELORIS_PELORIS II - Manual (Page 100)

Settings & Ancillary Operations
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 100
Additional limitations may apply depending on the retort state and the reagent in the retort. You
cannot raise the retort temperature above the safe temperature threshold of the reagent.
Wax Transfers
The wax path (comprising the wax valves and the transfer pipes) and the retort must be at the wax
standby temperature before you attempt to fill a retort with wax. Use the Wax heater buttons for
at least five minutes to enable wax path heating. Use the Temp. button to set the retort to the wax
standby temperature and wait until the retort reaches the temperature before filling.
Filling and Draining Retorts
Fill or drain the retorts from any reagent station using commands on the Manual operations
screen. The fill and drain functions operate with a series of rules designed to avoid reagent
contamination, reagent overflow spills and reagent overheating. You can override some rules but
this will often result in reduced reagent concentration.
The rules for manually filling and draining retorts are as follows.

The retort must be clean or empty before you commence a retort fill operation.

When filling a retort, the selected reagent station must be compatible with the retort residue.
See 8.5 Reagent Compatibility Tables.

You cannot fill a retort with a reagent that has a temperature threshold below the set retort
temperature.

When draining a retort, the reagent should return to its original station.

You cannot drain wax to a reagent bottle or non-wax reagents to a wax station.

When draining a retort, the station must have sufficient capacity for the retort contents.
Always ensure the retort is in a suitable state before filling. To fill, select the retort and reagent
station then tap Fill retort.
To drain a retort select the retort and the station the retort contents came from. Tap the Drain
retort button. The system will not allow you to drain a retort to a station other than the one the
reagent came from.
You can stop a fill or drain at any time with the Abandon button. Recover from an abandoned fill
or drain by restarting the operation, or return the reagent to the station or retort it came from with
the opposite operation.
WARNING
Do not open a retort if it has been filled with hot reagent and pressurized using the manual
operations functions. Always use the manual operations functions to de-pressurize a retort
before opening and wear suitable protective clothing (including eye protection) if the retort
contains hot reagent.