Leica Biosystems PELORIS_PELORIS II - Manual (Page 131)

Reference
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 131
8.2.4 Xylene-free Protocols
All the xylene-free protocols below are designed for use with the xylene-free bottle configuration in
8.3 Station Configurations.
Xylene-free 1 Hour

Pre-defined protocol “Factory 1 hr Xylene Free“

Carryover setting in pre-defined protocol: 100
Xylene-free 2 Hour

Pre-defined protocol “Factory 2 hr Xylene Free“

Carryover setting in pre-defined protocol: 75
Step Reagent type Time (min) Temp (°C) P/V Stirrer
Drip time
(s)
1 Formalin 1 Ambient Ambient Medium 10
2 85% Ethanol 1 Ambient Ambient Medium 10
3 85% Ethanol 6 55 Ambient Medium 10
4 80/20 Ethanol/IPA 1 Ambient Ambient Medium 10
5 80/20 Ethanol/IPA 6 55 Ambient Medium 10
6 IPA 1 Ambient Ambient Medium 10
7 IPA 1 Ambient Ambient Medium 10
8 IPA 12 55 Ambient Medium 10
9 Wax 20 85 Vacuum Medium 10
10 Wax 5 85 Vacuum Medium 10
11 Wax 1 65 Vacuum Medium 10
Processing time 1:19:00
Step Reagent type Time (min) Temp (°C) P/V Stirrer
Drip time
(s)
1 Formalin 1 Ambient Ambient Medium 10
2 85% Ethanol 1 Ambient Ambient Medium 10
3 85% Ethanol 12 55 Ambient Medium 10
4 80/20 Ethanol/IPA 1 Ambient Ambient Medium 10
5 80/20 Ethanol/IPA 25 55 Ambient Medium 10
6 IPA 1 Ambient Ambient Medium 10
7 IPA 1 Ambient Ambient Medium 10
8 IPA 25 55 Ambient Medium 10
9 Wax 25 85 Vacuum Medium 10
10 Wax 10 85 Vacuum Medium 10
11 Wax 5 65 Vacuum Medium 10
Processing time 2:11:00