Leica Biosystems PELORIS_PELORIS II User Manual
Page 41
Running Protocols
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011
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2. Check that the instrument is ready:
(i) All 16 bottles are properly installed on the instrument.
(ii) There is enough reagent in the bottles and wax chambers.
Fill to at least the MIN 2 level if you fill retorts to the two-basket level, or at least the
MIN 3 level for the three-basket level (view the instrument fill level in the Reagent fill
levels section on the Instrument settings screen).
(iii) Open the retort to use. Check that it is clean, or that any residue is a reagent compatible
with the first reagent in the protocol.
3. Prepare your cassettes and load them into baskets (see 2.2.4 Cassette Baskets).
Load Protocol
4. Tap the Select button at the bottom left or right corner of the Status screen, depending on
the retort you want to use.
The Protocol selection screen opens.
5. Select a protocol and tap Load.
Only validated protocols can be selected by operator-level users (see 4.1.4 Protocol Validation).
The Status screen reopens with the protocol loaded.
Run Protocol
6. Tap the Run button.
Review any warnings and rectify as required. If necessary, cancel the warnings dialog box
while you rectify conditions.
If the first step of the protocol uses a reagent that is incompatible with retort residue you may
need to abandon the protocol to run a cleaning protocol. Alternatively, you may be able to skip
the first steps of the protocol (see 3.4.2 Editing the Protocol for a Single Run). If so, tap
Continue in the warnings dialog box.
7.
If prompted, enter the number of cassettes in the run in the Number of cassettes dialog
box.
It is important to enter the correct number of cassettes – this is used by the reagent
management system to calculate reagent concentrations.
8. Open the retort lid and insert the cassettes when directed in the Insert cassettes dialog box.
Tap Done in the dialog box when you have inserted the cassettes and closed the retort.
9. View the protocol end-time in the Scheduling dialog box.
If acceptable, tap Start, or set a different end time (see 3.4.1 Scheduling Protocols).
Tap Edit steps in the Scheduling dialog box to skip initial protocol steps or change step
durations (see 3.4.2 Editing the Protocol for a Single Run).
10. When you tap Start the system schedules the protocol.
If the system is unable to schedule the protocol, you will see a list of errors. Tap OK and
correct all errors before attempting to run the protocol again (see 3.6.2 Unavoidable
Reagent Clashes for a description of possible scheduling difficulties).
If the system was able to schedule the run, the protocol will begin.
If you have set a delayed end time, an initial fill condition will occur (see 3.6.1 Delayed