Leica Biosystems PELORIS_PELORIS II - Manual (Page 99)

Settings & Ancillary Operations
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 99
6.1.1 Manual Operations
The Manual operations screen (Reagents menu, Manual operations) allows you to manually
control the instrument. The screen displays the current instrument status including station states,
the retort states and the retort condition. You control the instrument by selecting instrument
elements from the status area and control functions from the panels on either side.
All users can perform manual operations functions.
You can fill a retort from any station and then set conditions in the retort: temperature, pressure
and stirrer. If intending to move wax into a retort preheat the wax path by turning on the wax
heater. Use the wax vent function to neutralize the pressure in the wax bath so the wax bath lids
can be opened easily and safely.
You cannot override a running protocol from the Manual operations screen and you cannot fill or
drain a retort that has a loaded protocol.
Figure 58 shows the Manual operations screen with function buttons explained:
Figure 58. Manual operations screen
Retort Temperature Range
The retort temperature range is limited to the following values.

Reagent — 35 ºC minimum to 85 ºC maximum (restricted to 65 ºC maximum when running
tissue processing protocols).

Wax — (Wax melting point + 2 ºC) minimum to 85 ºC maximum (restricted to 77 ºC when
running standard protocols).
View the current wax melting point temperature on the Reagent management screen.
Fill/Drain retort:
fill or drain the retort
from or to the selected
station
Stirrer: set the retort
stirrer to high, medium
or low speed, or off
Temp.:
set the retort
temperature
Retort A controls
Wax heater: 
turn on/off heaters for
wax path
Pressure: 
set the pressure in the
retort – ambient,
vacuum, pressure, or
pressure/vacuum cycle
Abandon: 
stop a fill or drain
operation
Retort B controls
Vent wax:
release pressure or
vacuum in wax chambers