Leica Biosystems PELORIS_PELORIS II - Manual (Page 59)

Protocol Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 59
Other protocol properties are the reagent selection method and a carryover value that must be set
for each protocol. These, and protocol validation, pre-defined protocols and file saving are
described in the sections below.

4.1.1 Protocol Types

4.1.2 Reagent Selection Method

4.1.3 Pre-defined Protocols

4.1.4 Protocol Validation

4.1.5 Carryover Setting

4.1.6 Saving Protocol Files
4.1.1 Protocol Types
The Peloris tissue processor uses five protocol types for different processing functions. The protocol
types allow different reagent sequences and temperature ranges (see 8.5 Reagent Compatibility
Tables and 8.4 Protocol Step Temperatures). Once a protocol is created you cannot change its type.
The protocol types are:

Standard — conventional tissue processing sequences using a clearer such as xylene. Suitable
for normal processing requirements. These protocols can be constructed with or without
defatting steps.

Standard reprocessing — to recover under-processed tissue on an instrument configured for
standard processing. These protocols start with cleaning reagents before moving to a standard
tissue processing sequence.

Xylene-free — protocols with high temperature wax steps and advanced processing
techniques to process tissue without conventional clearing steps. Suitable for normal
processing requirements.

Xylene-free reprocessing — to recover under-processed tissue on an instrument configured
for xylene-free processing. These protocols start with cleaning reagents before moving to a
xylene-free tissue processing sequence.

Cleaning — protocols to clean the retorts and reagent lines. Always run a cleaning protocol as
soon as possible after wax has been in the retort. See 3.2 Cleaning Protocols for further
information.
See Protocol Icons in 4.2.1 The Protocol Selection Screen for the icons used for each protocol type.
Note the following points:

Xylene-free reagent selection — xylene-free protocols use two sets of dehydrants rather
than dehydrant followed by clearer, as used in standard protocols. This means xylene-free
protocols cannot use group reagent selection (see 4.1.2 Reagent Selection Method).

Xylene-free baskets — always use spaced cassette baskets for xylene-free protocols.

Reprocessing carryover — during reprocessing protocols there is significant reagent carry-
over following the cleaning stages. After running a reprocessing protocol you should replace
the first three processing reagents that are used after the last cleaning reagent.