Leica Biosystems PELORIS_PELORIS II - Manual (Page 130)

Reference
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 130
Xylene 12 Hour

Pre-defined protocol “Factory 12hr Xylene Standard“

Carryover setting in pre-defined protocol: 25
Step Reagent type
Reagent
group
Time
(min)
Temp
(°C)
P/V Stirrer
Drip time
(s)
1 Formalin Fixatives 44 45 Ambient Medium 10
2 Ethanol Dehydrants 30 45 Ambient Medium 10
3 Ethanol Dehydrants 30 45 Ambient Medium 10
4 Ethanol Dehydrants 30 45 Ambient Medium 10
5 Ethanol Dehydrants 30 45 Ambient Medium 10
6 Ethanol Dehydrants 60 45 Ambient Medium 10
7 Ethanol Dehydrants 90 45 Ambient Medium 10
8 Xylene Clearers 45 45 Ambient Medium 10
9 Xylene Clearers 45 45 Ambient Medium 10
10 Xylene Clearers 90 45 Ambient Medium 10
11 Paraffin wax Wax 60 65 Vacuum Medium 10
12 Paraffin wax Wax 60 65 Vacuum Medium 10
13 Paraffin wax Wax 80 65 Vacuum Medium 10
Processing time 12:02:00