Leica Biosystems PELORIS_PELORIS II - Manual (Page 77)

Reagent Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 77
Stations with reagent that has exceeded its change threshold are shown with a hatched
icon on the Status screen (bottle icon shown at right). In addition, the system displays a
warning message.
The Peloris system does not use out-of-change-threshold stations unless no other stations
are available. If no other stations are available out-of-threshold stations will be used for one
run (default setting) before being locked. Locked stations cannot be used at all until the
reagent is replaced. The system will not allow you to load protocols that require a locked
station.
Final Reagent Thresholds
Final reagent thresholds set limits on reagent purity for use in protocol steps that precede a change
to another reagent group (or type, for protocols configured by type), in the next step.
Final reagent thresholds are set higher than change thresholds. This ensures minimum
contamination from the preceding reagent group to the subsequent reagent group.
Reagents that have exceeded their final threshold are displayed as usual on the Status screen,
however Peloris displays a warning message. Following the warning you are able to process one run
(default setting) that uses reagent that is out of threshold for the final step. After this, you will not
be able to load protocols that require the reagent group affected.
When you are warned that reagent has exceeded the final threshold replace the least pure bottle of
the reagent type concerned. The bottle that exceeded the final reagent threshold, triggering the
warning, will still have reagent with a relatively high concentration. It will still be acceptable for
steps in the protocol sequence before the final step, so it is inefficient to replace it.
Temperature Thresholds
There are three temperature thresholds for each reagent type:

Ambient — the highest allowable temperature for the reagent in the retort at ambient (and
high) pressure

Vacuum — the highest allowable temperature for the reagent in the retort when the retort is
evacuated

Safe — the highest temperature at which it is safe to open a retort containing the reagent.
The Peloris software will not allow you to create protocols that put reagents in conditions that
exceed their ambient or vacuum temperature thresholds. The software warns you if you perform an
action that requires you to open a retort if the retort contains reagent above its safe temperature
threshold.
WARNING
Take extreme care if changing reagent temperature thresholds. Raised thresholds can lead to
reagents boiling and/or the release of large quantities of fumes that can overload the filtering
system. Boiling reagents can produce excessive pressures within the instrument increasing the
chance of reagent contamination and spills.