Leica Biosystems PELORIS_PELORIS II - Manual (Page 60)

Protocol Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 60
4.1.2 Reagent Selection Method
All protocols use one of three reagent selection methods: group, type or station (see 5.1.1 Reagent
Groups, Types and Stations for definitions of these terms). Once a protocol is created you cannot
change its reagent selection method.
A protocol’s reagent selection method determines the way the system selects reagent stations when
the protocol is run. With the station selection method you define exactly the station to use for each
step, so the system makes no “choices”. For group and type selection the system selects a station
from a number that are available to it, to get the best concentration for the protocol step (see
Station Selection Rules below). In summary:

Group selection — the system selects from all stations with reagent of the group defined for
the protocol step. A station’s reagent name (its type) is not used for selection, only the reagent
group and concentration.
For example, if you have bottles with reagent types “Ethanol 70%” and “Ethanol 90%” on the
instrument, the system will pick an “Ethanol 90%” bottle for the first dehydrant step if that
bottle has the lowest (in-threshold) concentration. Both reagent types are dehydrants, so are
equal candidates for dehydrant steps.

Type selection — the system selects from all stations with reagent of the type defined for the
protocol step. Station reagent names (i.e. types) and concentration are used for selection.
Using the example above, with “Ethanol 70%” and “Ethanol 90%” bottles on the instrument, if
the first dehydrant step in the protocol specifies “Ethanol 70%” then the Ethanol 70% bottle
with the lowest (in-threshold) concentration will be used, even if there is an Ethanol 90%
bottle with lower concentration. Because Ethanol 70% and Ethanol 90% are different reagent
types the system does not consider them both.

Station selection — the system uses the stations defined in the protocol (typically, users
define the bottles in order: bottle 1 first, then bottle 2, 3, etc.). Stations are defined by number,
so the name of the reagent in a station (its type), nor the reagent group are used for selection.
Again using the example above, if there are two Ethanol 70% bottles on the instrument and
the first dehydrant step of the protocol is configured to the first of these bottles, that bottle will
be used regardless of the concentration of reagent in the other bottle.
Station Selection Rules
The system uses the following rules to select stations for protocols with group and type reagent
selection. “Sequence”, as used below, means a series of protocol steps using the same reagent
group or reagent type.

The first step of a sequence uses the lowest concentration station available.

The last step of a sequence uses the highest concentration station available.

Intermediate steps in a sequence use the lowest concentration station that has not already
been used.

Where there is a single step for a particular reagent group or type the highest concentration
station is used.

Stations that have exceeded any of their use thresholds (and are not locked out) are not
selected unless there is no other station available.