Leica Biosystems PELORIS_PELORIS II - Manual (Page 132)

Reference
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 132
Xylene-free 4 Hour

Pre-defined protocol “Factory 4 hr Xylene Free“

Carryover setting in pre-defined protocol: 50
Xylene-free 6 Hour

Not a pre-defined protocol
Step Reagent type Time (min) Temp (°C) P/V Stirrer
Drip time
(s)
1 Formalin 10 55 Ambient Medium 10
2 85% Ethanol 3 Ambient Ambient Medium 10
3 85% Ethanol 22 55 Ambient Medium 10
4 80/20 Ethanol/IPA 10 Ambient Ambient Medium 10
5 80/20 Ethanol/IPA 40 55 Ambient Medium 10
6 IPA 3 Ambient Ambient Medium 10
7 IPA 10 55 Ambient Medium 10
8 IPA 45 55 Ambient Medium 10
9 Wax 45 85 Vacuum Medium 10
10 Wax 20 85 Vacuum Medium 10
11 Wax 10 65 Vacuum Medium 10
Processing time 4:02:00
Step Reagent type Time (min) Temp (°C) P/V Stirrer
Drip time
(s)
1 Formalin 20 55 Ambient Medium 10
2 85% Ethanol 15 55 Ambient Medium 10
3 85% Ethanol 20 55 Ambient Medium 10
4 80/20 Ethanol/IPA 20 55 Ambient Medium 10
5 80/20 Ethanol/IPA 45 55 Ambient Medium 10
6 IPA 15 55 Ambient Medium 10
7 IPA 30 55 Ambient Medium 10
8 IPA 60 55 Ambient Medium 10
9 Wax 45 85 Vacuum Medium 10
10 Wax 40 85 Vacuum Medium 10
11 Wax 30 65 Vacuum Medium 10
Processing time 6:04:00