Leica Biosystems PELORIS_PELORIS II - Manual (Page 94)

Reagent Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 94
5.4.4 Replacing Reagent – Manual
To replace bottles manually remove the bottles and dispose of the old reagent following your
laboratory’s standard procedures. Clean the bottle if necessary, and then fill with fresh reagent.
Load back on to the instrument.
When the bottle is reinserted the Inserted bottle configuration dialog box opens, showing the
reagent type, concentration and use history for either:

the bottle when it was removed, or

the bottle as configured in the Reagent stations screen, if you assigned a new reagent type
to the station after the bottle was removed (see Assigning New Reagents to Stations).
Figure 56. Inserted bottle configuration dialog box
The bottle state is set to Unknown in the dialog box.
Tap the table row and select one of the options at the bottom of the dialog box:

Changed — select if you replaced the old reagent with fresh reagent.
With this option the software prompts you to accept the default concentration for the reagent
in the station. If you know the concentration to be other than the default you can enter a
different concentration.
The use history values are all set to zero.
If you changed the reagent type in the bottle you cannot update it in the software from this
dialog box. Tap No change and then remove the bottle again. Change the reagent assigned to
the bottle in the Reagent stations screen (see Assigning New Reagents to Stations), then
reinsert the bottle.

Topped off/up — select if you did not change all the reagent but added a small amount of
fresh reagent of the same type to bring up its level in the bottle.
With this option the bottle state changes to Full. The concentration and use history details do
not change.

No change — select if you made no changes to the reagent in the bottle at all.
Tap in the table row to
select the bottle, then
select an option button
below
No change:
select if you did not do
anything to the
reagent in the bottle
Topped off/up:
select if you added a
small amount of reagent
to the bottle to bring up
its level
Changed: 
select ONLY if you replaced
the bottle contents with
fresh reagent