Leica Biosystems PELORIS_PELORIS II - Manual (Page 126)

Reference
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 126
8.2 Protocols
The protocols in this section have been developed and extensively tested by Leica Microsystems for
use on the Peloris tissue processor. Some are included as pre-defined protocols with all Peloris
systems.
When used for the recommended tissue types the protocols all produce optimum processing quality
with consistent, high-quality results. Use these protocols and the suggested station configurations
(see 8.3 Station Configurations) as a reference point when developing protocols that suit your
specific requirements and practices.
This section has the following subsections:

8.2.1 Specimen Type and Protocol Duration

8.2.2 List of Pre-defined Protocols

8.2.3 Xylene Protocols.

8.2.4 Xylene-free Protocols

8.2.5 Cleaning Protocol
8.2.1 Specimen Type and Protocol Duration
The following table lists recommended protocol durations for different specimen types.
8.2.2 List of Pre-defined Protocols
Leica Microsystems provides 11 pre-defined protocols with each Peloris system. You can use these
as they are, or base new protocols on them (see Copying Protocols in 4.2.3 Creating New Protocols
for instructions to copy pre-defined protocols).
Descriptions of the pre-defined protocols are included in the following sections.
Duration
(hours)
Maximum tissue
dimensions (mm) Specimen type
1 1.5 Endoscopies and needle biopsies
2 <3 All biopsies up to 3 mm diameter: gastrointestinal biopsies, renal,
prostatic, hepatic and breast cores; punch biopsies of skin, small
colonic polyps
4 3 Small specimens of non-dense tissues (e.g. kidney, liver, bowel),
excisional and incisional skin biopsies; skin ellipses
6 15 x 10 x 3 All routine tissues up to maximum dimensions (excluding fatty or
brain specimens)
8 15 x 10 x 4 All routine tissues up to maximum dimensions (excluding fatty or
brain specimens)
12 20 x 10 x 5 All routine tissues up to maximum dimensions. Very thick fatty
specimens may require a longer protocol.