Leica Biosystems PELORIS_PELORIS II - Manual (Page 76)

Reagent Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 76
5.1.3 Thresholds
Each reagent type has a range of thresholds that supervisors can configure on the Reagent types
screen. The thresholds for the pre-defined reagent types should be adequate for most laboratories,
but alternative settings may suit some laboratories better – contact customer support for advice
before changing threshold settings. Validate any threshold changes using the procedures that apply
to your laboratory.
The thresholds fall into two categories:

Purity thresholds set usage limits for reagents according to their purity

Temperature thresholds set limits on the retort temperatures for reagents to help ensure high
processing quality and safety.
Purity Thresholds
Peloris uses purity thresholds to limit the use of reagent as it becomes increasingly contaminated
with reagent carried over from other groups.
When a threshold is exceeded the software warns you that you need to replace reagent. Under
default settings you are able to use a station with out-of-threshold reagent for one more run after
such a warning. Following this the station is locked (meaning it cannot be used at all), and/or you
cannot run further protocols until fresh reagent is loaded. Your customer support representative can
change the number of runs allowed after reagent replacement warnings.
Reagent purity is assessed using one or more of four different “reagent threshold check” methods:

Concentration of the reagent

Number of cassettes processed with the reagent

Number of processing runs (cycles) the reagent has been used for

Number of days the reagent has been loaded on the instrument
By default, all of these methods are available for you to configure for individual reagent types. The
Reagent threshold check section of the Reagent management screen shows the methods
enabled for your system (these settings can only be changed by customer support representatives).
The checking methods are configured to monitor two types of purity threshold:

reagent change thresholds, and

final reagent thresholds,
explained below.
Reagent Change Thresholds
Reagent change thresholds set the limits for any use of reagent on the Peloris system. Reagent that
has exceeded its change threshold should be replaced with fresh reagent immediately.