Rapid reverse cleaning protocol below). do, See slow reverse cleaning protocol below – Leica Biosystems PELORIS_PELORIS II User Manual
Page 152
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Troubleshooting
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011
152
Rapid Reverse Cleaning Protocol
Slow Reverse Cleaning Protocol
Step Reagent type
Time
(min)
Temp (°C)
P/V
Stirrer
Drip
time (s)
1
Cleaning solvent
12
65
Ambient
High
10
2
Cleaning ethanol
6
55
Ambient
High
10
Processing time
18
Step Reagent type
Time
(min)
Temp (°C)
P/V
Stirrer
Drip
time (s)
1
Cleaning solvent
60
65
Ambient
High
10
2
Cleaning solvent
60
65
Ambient
High
10
3
Cleaning ethanol
60
45
Ambient
High
10
4
Cleaning ethanol
60
45
Ambient
High
10
Processing time
240
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