Leica Biosystems PELORIS_PELORIS II - Manual (Page 74)

Reagent Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 74
Reagent type names do not affect reagent concentration. For example, when assigning a reagent
type called “Ethanol 70%” to a station, the initial concentration value would be the type’s default
value (probably 70%) but you could set the initial concentration to anything between 0 and 100%.
Stations
The Peloris tissue processor has 20 reagent stations: the 16 reagent bottles and the 4 wax
chambers.
Each station has the following properties:

The reagent type that the station contains

The concentration of the reagent in the station, as calculated by the Peloris system

The station’s use history:

The number of cassettes processed with the reagent in the station

The number of runs (cycles) processed with the reagent in the station

The number of days the reagent has been in the station

The station’s state:

Dry: the station has been completely drained leaving only a small amount of residue. It
can be filled with any reagent.

Empty: reagent has been removed from the station to fill a retort. Reagent in excess of
the amount needed to fill the retort remains in the station.

In use: a reagent transfer is in progress or has been abandoned.

Full: the station holds enough reagent to fill a retort.

Not molten: for wax chambers only, the state to set when adding solid wax (see 5.4.5
Replacing Wax).

For wax chambers only, the chamber’s current temperature.
Use the Reagent stations management screen to define reagent stations and to monitor their
use history and concentration (see 5.3 Managing Reagent Stations).
5.1.2 Concentration Management
The high quality tissue processing delivered by the Peloris system is largely due to its accurate
monitoring of the concentration of the reagents in each station. This section describes the main
features of concentration management in the system.
Concentration in the Peloris System
Concentration is the proportion of a reagent that is of the group to which the reagent is assigned.
The following examples illustrate how the concentration is determined.

A dehydrant that is 80% ethanol (a dehydrant) and 20% water (not a dehydrant) has a
concentration of 80%.

A dehydrant that is 80% ethanol (a dehydrant) and 20% IPA (also a dehydrant) has a
concentration of 100%.