1 reagent threshold guidelines, Reagent threshold guidelines – Leica Biosystems PELORIS_PELORIS II User Manual
Page 125
Reference
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011
125
8.1 Reagent Threshold Guidelines
The tables in this section list the recommended thresholds for commonly used reagents. There are
separate tables for xylene and xylene-free processing:
Xylene Processing
For the best results, xylene-processing reagent changes should be based on concentration
thresholds, while cleaning reagent thresholds should be based on cycles.
Thresholds for xylene-processing reagents, including cleaning reagents, using nongraded alcohol
are:
Xylene-free Processing
In general, xylene-free processing reagent changes should be based on concentration thresholds,
and cleaning reagent changes based on cycles.
* This differs from the recommendation for cleaning ethanol in xylene processing because the cleaning solvent
in xylene-free protocols is less efficient than the cleaning solvent in xylene protocols.
Reagent change
thresholds
Final reagent
thresholds
Max. temperatures
C
Type
Conc. (%) Cassettes
or cycles
Conc. (%) Cassettes
or cycles
Ambient Vacuum Safe
Formalin
98.0
1500 cass. N/A
N/A
60
60
45
Ethanol
51.0
N/A
98.0
1500 cass. 78
51
45
Xylene
68.0
N/A
95.0
1500 cass. 138
99
45
Wax
80.0
4500 cass. 95.0
1500 cass. 100
100
77
Cleaning xylene
88.0
10 cycles N/A
N/A
138
99
45
Cleaning ethanol
88.0
10 cycles N/A
N/A
78
51
45
Reagent change
thresholds
Final reagent
thresholds
Max. temperatures
C
Type
Conc. (%) Cassettes
or cycles
Conc. (%) Cassettes
or cycles
Ambient Vacuum Safe
Formalin
98.0
1500 cass. N/A
N/A
60
60
45
85% ethanol
50.0
N/A
N/A
N/A
87
55
45
80/20 ethanol/IPA
81.0
N/A
N/A
N/A
78
51
45
IPA
90.0
4500 cass. 95.0
1500 cass. 82
55
45
Wax
85.0
4500 cass. 95.0
1500 cass. 100
100
77
Cleaning Waxsol
88.0
6 cycles* N/A
N/A
100
100
45
Cleaning ethanol
88.0
6 cycles* N/A
N/A
78
51
45