Retorts, Wax bath settings, Global wax settings – Leica Biosystems PELORIS_PELORIS II User Manual

Page 102

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Settings & Ancillary Operations

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011

102

Retorts

The Retorts section of the Reagent management screen shows the state of and last reagent

used for each retort. It also shows the empty access temperature – the temperature up to which it

is safe to open empty retorts. The system warns you if you perform any action that requires you to

open an empty or clean retort with a temperature exceeding this value.

The settings in this section can only be changed by your service representative.

Wax Bath Settings

Turn wax cleaning on and off with the Wax clean button on the Reagent management screen.

The wax clean function improves the quality of the wax by periodically evacuating the wax bath (for

approximately 30 seconds) to draw off any contaminants. You may need to periodically add small

amounts of wax to replace solvents that have been removed.

Enable wax cleaning for protocols that use xylene and/or IPA.

Disable wax cleaning for protocols that use xylene or IPA substitutes – these clearers are not

efficiently removed by the evacuation process.

By removing contaminants wax cleaning increases wax concentrations. The system automatically

updates the concentration of each wax station after each wax clean cycle.

Wax cleaning occasionally delays the start of a protocol or other action for up to 30 seconds.

Global Wax Settings

Set the wax melting and wax standby temperatures in the Global settings section of the

Reagent management screen.

The wax standby temperature is the temperature the instrument heats the wax to in the wax

stations. The default temperature is 65 ºC.

The wax melting point is the temperature at which the instrument considers the wax molten.

The default temperature is 56 ºC.

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