Leica Biosystems PELORIS_PELORIS II - Manual (Page 64)

Protocol Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 64
run uses the respective cassette or pad type.
Sample Calculation
The average run of a protocol uses the following proportions of tissue carriers:

Surgipath ActivFlo Routine I: 40%

Surgipath Biopsy Wraps in Surgipath ActivFlo Routine I: 60%
Multiply the proportion of each carrier with its value from the table above to calculate the additional
carryover value for that carrier:

Surgipath ActivFlo Routine I: 40% × 0 = 0

Surgipath Biopsy Wraps in Surgipath ActivFlo Routine I: 60% × 20 = 12
Add the resulting values:

0 + 12 = 12
Enter a carryover value of 12 for the protocol.
Peloris software versions before version 1.40 had a “Biopsy pad” setting for small-tissue carrier
carryover. Users with satisfactory “Biopsy pad” settings in those versions should use the same
numeric value for the Carryover setting.
Other Products
For small-tissue carriers other than those in the table above we recommend you start with a
carryover setting of 50. Use your own observations of tissue and reagent quality to refine this
value. Tissue shrinkage in the block and excess solvent in the wax bath might both be indications
that you have too low a carryover setting.
It is users’ responsibility to validate the carryover settings in their protocols.
Contact your technical support representative if you require assistance setting suitable carryover
settings.
Leica Microsystems products Generic description of tissue carrier
Carryover value if
100% of tissue uses
carrier
Surgipath ® ActivFlo ™ Routine I Standard cassette 0
Surgipath Biopsy Pads in 
Surgipath ActivFlo Routine I
Biopsy pad in standard cassette 100
ActivFlo Mini Cassette in 
Surgipath ActivFlo Routine I
Mini cassette in standard cassette 50
Surgipath Biopsy Wraps in 
Surgipath ActivFlo Routine I
Biopsy wrap in standard cassette 20
Surgipath IP ActivFlo Biopsy I 
McCormick ™ MC-605
Biopsy cassettes with >1 mm pore size 0