Leica Biosystems PELORIS_PELORIS II - Manual (Page 92)

Reagent Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 92
4. When prompted, check that the retort lid is closed and the remote fill/drain hose is properly
connected.
Tap OK to begin the drain.
5. The instrument will now drain the bottle(s) via the selected retort.
Monitor the drain progress in the status panel.
When the drain completes, the retort state will be Empty and the bottle state Dry.
Fill
6. Place the hose into the container of fresh reagent.
The reagent source must be above 5 °C to ensure that all reagent sensors operate correctly.
7. From the Remote fill/drain screen, select:

The retort to use

The Remote icon

The bottle(s) to fill (multiple bottles must all be Dry and set to the same reagent type)
Any bottle residue must be compatible with the new reagent.
8. Tap Fill to bottle(s) to begin filling.
9. When prompted, check that the retort lid is closed and the remote fill/drain hose is properly
connected.
Tap OK to begin the fill.
10. The Remote fill/drain - Reagent details dialog box will now appear.
Figure 55. Reagent details dialog box with reagent type, concentration and
use history for the new reagent
11. Confirm that the reagent type, concentration and use history details are correct, or tap in the
table cells for any of these values to change the settings.
If you set a new reagent type the station must have already been set to that type (see
Assigning New Reagents to Stations in 5.3 Managing Reagent Stations). The reagent must be
compatible with the retort and bottle residue.
Tap OK to start the fill.
WARNING
Do not remove the remote fill/drain hose until the software indicates the process is complete
and pressurized air has cleared the hose. A cessation of reagent flow is not an indication that
the procedure is complete.