5 cleaning protocol, Cleaning protocol, Quick clean) – Leica Biosystems PELORIS_PELORIS II User Manual

Page 134

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Reference

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011

134

8.2.5 Cleaning Protocol

Quick Clean

Pre-defined protocol “Quick Clean“.

Bottle configuration: one bottle each of cleaning solvent and cleaning alcohol.

Never run a cleaning protocol while tissue is in the retort as the dry step will damage the

tissue.

Step Reagent group

Time (min) Temp (°C) P/V

Stirrer

Drip time

(s)

1

Cleaning solvents

12

75

Ambient

High

10

2

Cleaning alcohols

6

55

Ambient

High

10

3

Dry step

12

80

N/A

Off

N/A

Processing time

0:34:00

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