8 block artifact, Block artifact – Leica Biosystems PELORIS_PELORIS II User Manual

Page 148

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Troubleshooting

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011

148

9.2.8 Block Artifact

Soft ti

ssue

T

issue shrinkag

e

Swea

ti

ng ti

ssu

e

Insuffi

cie

nt

proce

ssing

ti

me

Inc

re

ase

proce

ssing

time

Adjust thresholds

In

correct

re

age

nt

thr

e

sh

o

ld

s

Dec

re

ase

tissue si

ze

wh

en

p

roc

essi

ng

Insu

fficient

cle

ar

in

g,

dehydration and/

or in

fi

lt

ra

ti

on

In

cr

ea

se p

rot

oc

ol

ste

p

ti

me

s

Increa

se

fi

xat

io

n

In

app

rop

ri

at

e

fi

xat

io

n

Insu

ff

ic

ien

t

de

hyd

rat

io

n

Incre

ase

de

hydr

at

io

n

st

ep

s

In

cre

ase

cl

ea

ri

ng

Insuffi

cie

nt

ti

ssu

e c

lea

ri

ng

Insuffi

cie

nt

dehydration

Opa

qu

e b

loc

k

Incr

ease dehydration

ste

ps

Inc

rea

se pro

ces

sin

g

Insuffi

cie

nt

pro

ces

sing

Alway

s

try

to e

n

sure

that pr

otocol l

ength

is

cor

rect

for the size

of the

tissue

E

n

su

re

re

ag

en

ts ar

e c

lea

n

an

d at

co

rr

e

ct

pu

ri

ty

E

n

su

re

r

e

a

g

en

ts

ar

e

cle

an

a

n

d

at

co

rr

ect

puri

ty

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