Leica Biosystems PELORIS_PELORIS II - Manual (Page 142)

Troubleshooting
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 142
9.2.2 Over-processed Tissue – Instrument Setup
Over-processed tissue
(cooked/ crispy/ burnt)
Yes
Was tissue
allowed to dry
out?
Check:
· How was the tissue submitted to the lab-
in formalin?
· Was tissue in solution between grossing
and processing?
· Was the protocol in dehydrant for too
long?
· Was the protocol in solvent for too long?
· Was tissue left in the retort in wax for too
long?
· Was the tissue left in the embedding
instrument too long?
Yes
No
No
No
No
No
Yes
No
Yes No
Stop
Well Processed Tissue
Yes
Yes
Yes
Go to 9.2.3 Under- or
Over-processed Tissue – Reagents
No
Are any error
codes present?
If a user warning, fix the
issue. If a service warning,
contact service
Retest. Is the
tissue processed
correctly?
Was the correct
protocol used?
Check:
· tissue size
· step times
· pressure and vacuum
· temperatures
· drip times
Are the settings appropriate?
Optimise the reagent using the pre-defined
protocols as a guideline.
Was the
correct carryover
value, number of
cassettes,
entered?
Enter correct values for Carryover and
cassettes for the reagent management
system to work correctly.
Retest. Is the
tissue processed
correctly?
Retest. Is the
tissue processed
correctly?
Retest. Is the
tissue processed
correctly?
Is the lab
workflow
appropriate?
Check:
· Transfer fluid (formal saline?)
· Fixation time
· Water introduced after processing?
· Quality of embedding wax
Are these all correct?
No
Retest. Is the
tissue processed
correctly?
Yes
Yes
Yes
No