Leica Biosystems PELORIS_PELORIS II - Manual (Page 128)

Reference
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 128
Xylene 2 Hour

Pre-defined protocol “Factory 2hr Xylene Standard“

Carryover setting in pre-defined protocol: 75
Xylene 4 Hour

Pre-defined protocol “Factory 4hr Xylene Standard“

Carryover setting in pre-defined protocol: 50
Step Reagent type
Reagent
group
Time
(min)
Temp
(°C)
P/V Stirrer
Drip time
(s)
1 Formalin Fixatives 1 Ambient Ambient Medium 10
2 Ethanol Dehydrants 1 Ambient Ambient Medium 10
3 Ethanol Dehydrants 1 Ambient Ambient Medium 10
4 Ethanol Dehydrants 1 Ambient Ambient Medium 10
5 Ethanol Dehydrants 1 Ambient Ambient Medium 10
6 Ethanol Dehydrants 11 45 Ambient Medium 10
7 Ethanol Dehydrants 30 45 Ambient Medium 10
8 Xylene Clearers 1 Ambient Ambient Medium 10
9 Xylene Clearers 1 Ambient Ambient Medium 10
10 Xylene Clearers 28 45 Ambient Medium 10
11 Paraffin wax Wax 5 65 Vacuum Medium 10
12 Paraffin wax Wax 5 65 Vacuum Medium 10
13 Paraffin wax Wax 20 65 Vacuum Medium 10
Processing time 2:14:00
Step Reagent type
Reagent
group
Time
(min)
Temp
(°C)
P/V Stirrer
Drip time
(s)
1 Formalin Fixatives 10 45 Ambient Medium 10
2 Ethanol Dehydrants 1 Ambient Ambient Medium 10
3 Ethanol Dehydrants 1 Ambient Ambient Medium 10
4 Ethanol Dehydrants 1 Ambient Ambient Medium 10
5 Ethanol Dehydrants 20 45 Ambient Medium 10
6 Ethanol Dehydrants 20 45 Ambient Medium 10
7 Ethanol Dehydrants 45 45 Ambient Medium 10
8 Xylene Clearers 1 Ambient Ambient Medium 10
9 Xylene Clearers 10 45 Ambient Medium 10
10 Xylene Clearers 45 45 Ambient Medium 10
11 Paraffin wax Wax 10 65 Vacuum Medium 10
12 Paraffin wax Wax 10 65 Vacuum Medium 10
13 Paraffin wax Wax 40 65 Vacuum Medium 10
Processing time 4:02:00