Leica Biosystems PELORIS_PELORIS II - Manual (Page 80)

Reagent Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 80
5.1.6 Reagent Compatibility
As tissue processing requires the use of incompatible reagents, the Peloris tissue processor
software uses a set of rules to ensure that only compatible reagents are allowed to mix. Reagent
mixing usually occurs when a reagent enters a retort of state Empty, containing residual amounts
of the previous reagent. This may happen as part of a protocol, during manual operations or during
remote and fill/drain procedures.
The software will not allow you to create a protocol that has an incompatible reagent sequence and
you are also prevented from mixing incompatible reagents during remote fill/drain procedures. You
cannot run a protocol where the first reagent is incompatible with the retort residue. You can
however load a protocol with an incompatible first step but the reagent sequence must be edited
during scheduling to select a new first step that is compatible with the retort residue (see 3.4.2
Editing the Protocol for a Single Run).
Reagent compatibility varies depending on the action or protocol being undertaken. Use the
reagent tables in 8.5 Reagent Compatibility Tables to check reagent compatibility before creating
protocols, conducting manual operations or initiating remote fill/drain procedures.
5.2 Managing Reagent Types
The Peloris software uses two lists of reagent types – an “active” list for reagents you use and a
“dormant” list with all reagent types configured in the system. Supervisors can edit the default
concentrations and purity and temperature thresholds (see 5.1.3 Thresholds) for active reagents.
They can easily move reagents on and off the active list, and create new reagent types. Operators
can only view the active list.
See sections below for editing and managing reagent types:

5.2.1 Pre-defined Reagents

5.2.2 Editing Active Reagent Types

5.2.3 Managing the Reagent Types Lists
5.2.1 Pre-defined Reagents
A number of reagent types are pre-defined in the Peloris system. You can edit the properties of
these reagent types but they can cannot be deleted. There is no requirement to use the pre-defined
types however – you can leave them on the dormant list.
If you change the properties of pre-defined reagent types you cannot use the software to
automatically return them to their original values. However the default values for many of the pre-
defined reagents are shown in 8.1 Reagent Threshold Guidelines.