Leica Biosystems PELORIS_PELORIS II - Manual (Page 86)

Reagent Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 86
5.3.2 Setting Reagent Station Properties
See sections:

Assigning New Reagents to Stations

Changing Reagent Concentration

Setting Station States
Assigning New Reagents to Stations
If you change the type of reagent loaded in a station you must change the reagent type assigned to
the station in the software.
Follow the steps below to change the reagent type for a station:
1. Remove the bottle (or drain the wax chamber) with the reagent type you are replacing.
2. In the Reagent stations screen tap in the station’s Type cell to open the Reagent
selection dialog box.
3. Select the new reagent type from the list.
The dialog box shows all the current active reagent types (see 5.2.3 Managing the Reagent
Types Lists).
4. When prompted, tap Yes to reset the station properties. This sets the use history counts to
zero and the station concentration to the default for the new reagent type.
5. Clean the bottle if necessary and fill with fresh reagent of the new type. Load the bottle back
onto the instrument (or fill the wax chamber).
6. For a bottle, in the Inserted bottle configuration dialog box, select the bottle in the table
and then tap the Emptied and refilled button.
For a wax chamber, set the station state to Full on the Reagent stations screen.
When you change the reagent type for a station on the Reagent stations screen you are always
prompted to reset the station’s concentration and use history. If you select No you will retain the
previous reagent’s concentration and use history. Use this option only if you are correcting a
previous error in the identification of the reagent in the station, and you are not actually changing
the station’s contents.
CAUTION
Altering reagent station configurations while protocols are running may cause abandoned
protocols.
WARNING
Always ensure that the reagents configured in the software are the actual reagents loaded on
the instrument. A station containing different reagent could damage tissue samples.