Leica Biosystems PELORIS_PELORIS II - Manual (Page 73)

Reagent Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 73
5.1.1 Reagent Groups, Types and Stations
The Peloris tissue processor manages reagents by groups, types and stations.
Groups
Groups specify reagent function. For example, the fixatives reagent group includes all reagents that
can be used as a fixative.
There are nine factory-defined groups. Each group has a color-code used in the Peloris software
and on bottle labels. Refer to the following table for a list of the groups and their colors and
functions.
The reagent group determines reagent compatibility (see 5.1.6 Reagent Compatibility).
Types
Reagent types are the specific reagents within each group, e.g. formalin, xylene, Waxsol. As well as
the chemical constituent, reagent type definitions can include concentrations. For example “70%
Ethanol” and “80% Ethanol” are reagent types defined in Peloris.
Reagent types have the following properties:

A unique name

Default concentration: the concentration of the reagent when fresh.

Purity thresholds: to ensure you replace degraded reagent (see 5.1.3 Thresholds).

Temperature thresholds: used to ensure processing quality and safe usage of the reagent (see
5.1.3 Thresholds).
The Peloris software includes a number of pre-defined reagent types. These are adequate for most
laboratories, however you can create your own reagent types if needed.
Use the Reagent types screen to define and edit reagent types (see 5.2 Managing Reagent
Types).
Group Function Color
Fixatives Tissue preservative. Green
Dehydrants Removes fixative reagents from tissue. Blue
Defat Removes fat deposits from tissue. Yellow
Post defat The dehydrant used after a defatting step. Purple
Clearers Clears the dehydrants from the tissue. Pink
Wax The embedding medium. Orange
Cleaning solvents First cleaning reagent. Light pink
Cleaning alcohols Second cleaning reagent. Light blue
Cleaning water Third retort cleaning reagent. Grey