Leica Biosystems PELORIS_PELORIS II - Manual (Page 32)

Hardware
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 32
2.3 Wax Bath
The wax bath at the top rear of the instrument has four wax chambers accessed by two lids. Each
chamber acts independently, holding enough wax to fill a retort. Although wax does not move
between the chambers, they are connected for air flow and so always have the same pressure.
Use the lid handles to open the lids. Always use the handles to close the lids, ensuring the lids are
properly latched. Be sure not to knock the lids when they are open as they may slam shut.
Figure 16. Opening a wax bath lid
Always ensure the wax bath pressure is at ambient pressure before opening the lids. If the pressure
is not ambient, vent the bath first. You can do this when you pause a running protocol or from the
Manual operations screen.
View the current temperature of each wax station in the wax chambers view of the Reagent
stations screen.
Depending on the reagents you use you can set the instrument to clean the wax in the bath (see
Wax Bath Settings in 6.1.2 Reagent Management).
Drain used wax with commands on the Remote fill/drain screen. Directly fill with molten or solid
wax (see 5.4.5 Replacing Wax).
WARNING
Take care when opening the wax bath after a xylene-free protocol as the protocol will leave very
hot wax in the bath.
WARNING
Never open a wax bath lid when there is wax in a retort or wax is being transferred; hot wax
may splash out of the bath.
WARNING
Take care not to knock wax bath lids when they are open. They could crush fingers or hands.