Leica Biosystems PELORIS_PELORIS II - Manual (Page 140)

Troubleshooting
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 140
These questions should help you determine what has gone wrong. If you remain uncertain as to
the cause of the problem you must gather as much information as possible by carefully examining
the specimens. Close examination of the blocks can confirm what you suspect may have happened.
Microscopic examination will also provide valuable information to indicate what may have gone
wrong.
9.2 Flowcharts
This section has eight flowcharts for different processing problems.
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9.2.1 Under-processed Tissue – Instrument Setup
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9.2.2 Over-processed Tissue – Instrument Setup

9.2.3 Under- or Over-processed Tissue – Reagents

9.2.4 Poor Processing – Incorrect Protocol

9.2.5 Poor Processing – Correct Protocol
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9.2.6 Cutting Artifact
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9.2.7 Staining Artifact
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9.2.8 Block Artifact