Leica Biosystems PELORIS_PELORIS II - Manual (Page 58)

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 58
4
Protocol Setup
Protocols control all aspects of the tissue processing runs carried out on the Peloris. This chapter gives an
overview of Peloris protocols and how to create, edit and view protocols with the software interface.
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4.1 Protocol Overview
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4.2 Creating, Editing and Viewing Protocols
4.1 Protocol Overview
Protocols consist of sequences of steps applied to tissue in a retort. In each step (with one
exception) the retort is filled with reagent and held for a time under conditions controlled by the
protocol. Protocol steps have the following properties:

the reagent used

the time the reagent is in the retort

the temperature of the reagent

the retort pressure: ambient, pressurized or vacuum, or cycling between pressurized and
vacuum

the retort stirrer speed

the drip time: the time allowed for reagent to drip from the cassettes and retort walls before
ending a drain. Longer drip times reduce carryover.
The one step type that is an exception is the final “dry” step of a cleaning protocol, where no
reagent is used.
There are five protocol types. A protocol’s type sets limits on which reagents can be used and the
order of their use.