Leica Biosystems PELORIS_PELORIS II - Manual (Page 137)

Reference
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 137
8.4 Protocol Step Temperatures
The Peloris tissue processor uses five protocol types to accommodate different processing
functions. Each protocol type has a set of reagent compatibility sequences that comply with the
intended use (see 8.5 Reagent Compatibility Tables for more information). The allowable
temperature range for each step is also dependent on the protocol type. The following sections list
the protocol temperature ranges and typical protocol sequences.
The current wax melting temperature is shown on the Reagent management screen.
8.5 Reagent Compatibility Tables
The reagent compatibility tables determine the allowable reagent sequences. The sequences vary
depending on the operation or protocol type.
Manual Operations
Protocol type
Retort temp. range
for reagent
Retort temp. range for
wax
Wax bath temp.
range
Standard 35 ºC to 65 ºC 2 ºC above wax melt temp. 
to 77 ºC
55 ºC to 85 ºC
Xylene-free 35 ºC to 65 ºC 2 ºC above wax melt temp. 
to 85 ºC
55 ºC to 85 ºC
Cleaning 35 ºC to 85 ºC N/A 55 ºC to 85 ºC
Current step Previous step

= compatible
None Fixat
iv
es
Deh
ydr
ant
s
Clear
ers
Wa
x
Cleaning solv
ent
s
Cleaning alcohols
Fixatives    
Dehydrants      
Clearers     
Wax    
Cleaning solvents      
Cleaning alcohols      