Leica Biosystems PELORIS_PELORIS II - Manual (Page 127)

Reference
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 127
The pre-defined protocols are:

Factory 1hr Xylene Standard (see Xylene 1 Hour)

Factory 2hr Xylene Standard (see Xylene 2 Hour)

Factory 4hr Xylene Standard (see Xylene 4 Hour)

Factory 8hr Xylene Standard (see Xylene 8 Hour)

Factory 12hr Xylene Standard (see Xylene 12 Hour)

Factory 1 hr Xylene Free (see Xylene-free 1 Hour)

Factory 2 hr Xylene Free (see Xylene-free 2 Hour)

Factory 4 hr Xylene Free (see Xylene-free 4 Hour)

Factory 8 hr Xylene Free (see Xylene-free 8 Hour)

Factory 12 hr Xylene Free (see Xylene-free 12 Hour)

Quick Clean (see Quick Clean)
8.2.3 Xylene Protocols
All the xylene protocols below are designed for use with the xylene bottle configuration in 8.3
Station Configurations.
Xylene 1 Hour

Pre-defined protocol “Factory 1hr Xylene Standard“

Carryover setting in pre-defined protocol: 100
Step Reagent type
Reagent
group
Time
(min)
Temp
(°C)
P/V Stirrer
Drip time
(s)
1 Formalin Fixatives 1 Ambient Ambient Medium 10
2 Ethanol Dehydrants 1 Ambient Ambient Medium 10
3 Ethanol Dehydrants 1 Ambient Ambient Medium 10
4 Ethanol Dehydrants 1 Ambient Ambient Medium 10
5 Ethanol Dehydrants 1 Ambient Ambient Medium 10
6 Ethanol Dehydrants 1 Ambient Ambient. Medium 10
7 Ethanol Dehydrants 18 45 Ambient Medium 10
8 Xylene Clearers 1 Ambient Ambient Medium 10
9 Xylene Clearers 1 Ambient Ambient Medium 10
10 Xylene Clearers 14 45 Ambient Medium 10
11 Paraffin wax Wax 2 65 Vacuum Medium 10
12 Paraffin wax Wax 1 65 Vacuum Medium 10
13 Paraffin wax Wax 14 65 Vacuum Medium 10
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