Leica Biosystems PELORIS_PELORIS II - Manual (Page 62)

Protocol Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 62
Pre-defined protocols can be copied to appear in the Protocol selection screen where they
become available for use. They can be copied as they are, even keeping the name unchanged, or
once copied you can modify them to suit your purposes. The original versions cannot be changed.
See Copying Protocols in 4.2.3 Creating New Protocols for directions to copy a pre-defined protocol.
In new Peloris systems the pre-defined protocols are all provided, ready for use, in the Protocol
selection screen. In case these versions are edited or deleted, however, the original versions
remain available under the Pre-defined icon accessed with the New button.
4.1.4 Protocol Validation
Supervisors creating or editing protocols (or copying pre-defined protocols) have the option to
“validate” them in the Peloris software. This serves as a sign that the protocols have passed the
laboratory’s validation tests and can be used for regular clinical processing. Supervisors can also
make valid protocols invalid.
Protocols that have been marked as valid can be run by operator-level users, while invalid protocols
cannot. Invalid protocols can be run by supervisors.
Icons for validated protocols have a green check, while the icons for invalid protocols have a red
cross:
Set protocol validity status on the Edit protocol screen, with the Validate protocol / Invalidate
protocol button.
4.1.5 Carryover Setting
The Carryover setting is a protocol setting for the additional carryover due to biopsy pads and
other small-tissue carriers.
This section has, first, a general overview of carryover in the Peloris system, then describes how to
use the Carryover setting in particular.
Carryover – Overview
When reagent drains out of a retort and is replaced with reagent from another station a certain
amount of the first reagent remains in the retort and mixes with the new reagent. The Peloris
reagent management system calculates the amount of this “carryover” and uses it to determine
reagent concentrations.
Figure 35. Icon for a valid protocol Figure 36. Icon for an invalid protocol