Leica Biosystems PELORIS_PELORIS II - Manual (Page 28)

Hardware
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 28
2.2.3 Magnetic Stirrer
Each retort has as magnetic stirrer that stirs the reagent or wax to ensure temperature consistency
and good tissue penetration. The stirrer is driven by a magnetically-coupled external motor. The
stirrer speed can be controlled for each protocol step and is easily removed for cleaning (see Clean
Retorts in 7.2 Daily Tasks).
Figure 6. Magnetic stirrer (1)
2.2.4 Cassette Baskets
There are two Peloris basket types: the configurable high-capacity basket that accommodates the
maximum number and type of cassettes; and the spaced basket that ensures optimal reagent flow.
The high-capacity cassette baskets accept most common cassette types and include configurable
dividers that allow for different cassette sizes and packing densities.
The spaced baskets include dividers that ensure optimum reagent flow with minimal carryover. This
basket type accepts 72 standard cassettes. Spaced baskets must be used for all xylene-free
protocols.
Cassette baskets stack into the retorts with the lids upwards and handles dropped flat. Make sure
the first basket sits flat on the rack on the pins at the bottom of the retort (Figure 8). Stack
additional baskets so they sit flat on the lower ones – crooked baskets can leave cassettes exposed,
damaging the tissue in them.
Figure 7. Placing a basket into a retort Figure 8. Retort pins