Leica Biosystems PELORIS_PELORIS II - Manual (Page 129)

Reference
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 129
Xylene 6 Hour

Not a pre-defined protocol
Xylene 8 Hour

Pre-defined protocol “Factory 8hr Xylene Standard“

Carryover setting in pre-defined protocol: 25
Step Reagent type
Reagent
group
Time
(min)
Temp
(°C)
P/V Stirrer
Drip time
(s)
1 Formalin Fixatives 15 45 Ambient Medium 10
2 Ethanol Dehydrants 15 45 Ambient Medium 10
3 Ethanol Dehydrants 15 45 Ambient Medium 10
4 Ethanol Dehydrants 15 45 Ambient Medium 10
5 Ethanol Dehydrants 15 45 Ambient Medium 10
6 Ethanol Dehydrants 30 45 Ambient Medium 10
7 Ethanol Dehydrants 45 45 Ambient Medium 10
8 Xylene Clearers 20 45 Ambient Medium 10
9 Xylene Clearers 20 45 Ambient Medium 10
10 Xylene Clearers 45 45 Ambient Medium 10
11 Paraffin wax Wax 30 65 Vacuum Medium 10
12 Paraffin wax Wax 30 65 Vacuum Medium 10
13 Paraffin wax Wax 45 65 Vacuum Medium 10
Processing time 6:08:00
Step Reagent type
Reagent
group
Time
(min)
Temp
(°C)
P/V Stirrer
Drip time
(s)
1 Formalin Fixatives 20 45 Ambient Medium 10
2 Ethanol Dehydrants 20 45 Ambient Medium 10
3 Ethanol Dehydrants 20 45 Ambient Medium 10
4 Ethanol Dehydrants 20 45 Ambient Medium 10
5 Ethanol Dehydrants 20 45 Ambient Medium 10
6 Ethanol Dehydrants 40 45 Ambient Medium 10
7 Ethanol Dehydrants 60 45 Ambient Medium 10
8 Xylene Clearers 30 45 Ambient Medium 10
9 Xylene Clearers 30 45 Ambient Medium 10
10 Xylene Clearers 60 45 Ambient Medium 10
11 Paraffin wax Wax 40 65 Vacuum Medium 10
12 Paraffin wax Wax 40 65 Vacuum Medium 10
13 Paraffin wax Wax 60 65 Vacuum Medium 10
Processing time 8:08:00