Leica Biosystems PELORIS_PELORIS II - Manual (Page 120)

Cleaning and Maintenance
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 120
7.3 Weekly Tasks
Empty Condensate Bottle
Empty the condensate bottle weekly. This is especially important if running xylene-free protocols,
which have more condensate.
Check Reagent Bottles
Check all bottles weekly. Note bottles that are becoming dirty. Clean the bottles when next
replacing reagent.
While bottles are removed wipe the interior of the reagent cabinet with 70% alcohol.
To clean bottles pour in a small amount of fresh reagent (i.e. the reagent the bottle is used for),
cap the openings, and shake. Use sealing caps from Leica Microsystems to seal the bottle. Empty
the bottle and check it is clean. If so refill and replace in the instrument (see 5.4 Replacing
Reagents for instructions to reset the station properties).
If the bottle is still stained use a bottle brush and laboratory detergent to clean. Rinse thoroughly
with water. Then prepare the bottle to refill with reagent:

For formalin and alcohol bottles (where the reagent is miscible with water) rinse with a small
amount of the bottle’s reagent to clear the water, then refill.

For clearer and cleaning solvent bottles (e.g. xylene, where the reagent is not miscible with
water), dry thoroughly before adding fresh reagent, or rinse with a small amount of alcohol
then a small amount of the bottle’s reagent before refilling.
Clean Wax Bath
Check the wax chambers weekly and note if they are becoming dirty. Clean the chambers when
next replacing the wax.
Clean the wax chambers while they are warm after draining spent wax out of the instrument. Never
attempt to clean a chamber while the wax from the chamber is in the retort.
WARNING
Wear suitable protective clothing and eyewear to avoid splashes when cleaning bottles.
WARNING
Ensure lids are tight and the bottles are correctly plugged into the manifold after replacing
reagent to avoid reagent spills.
CAUTION
Do not clean reagent bottles in an automatic dishwasher as they may be damaged