Leica Biosystems PELORIS_PELORIS II - Manual (Page 55)

Running Protocols
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 55
Emergency Stop and Abandon
After you have paused the instrument you can abandon all protocols with the Emergency stop
button. Note that this does not shut down or remove power from the instrument (see 2.1 Switching
On and Shutting Down).
Alternatively, abandon the protocol run in one retort only with the appropriate Abandon button.
Any protocol running in the other retort will resume.
When processing stops the Peloris goes through the same routines carried out at the end of a
normal protocol run. It prompts you to drain the retort (if full), remove cassettes, and run a
cleaning protocol.
After abandoning a protocol you may wish to run a reprocessing protocol to recover your cassettes.
In this case do not remove your cassettes or run a cleaning protocol when prompted to do so.
If the last reagent was a fixative you may skip the cleaning protocol as the residue will not prevent
you running a typical processing protocol. If you decide to run a cleaning protocol, set the first step
to a cleaning alcohol. Cleaning solvents are incompatible with fixatives.
Access Retort
You can access retorts in a paused instrument in order to add or retrieve samples.
To access a retort while the instrument is processing:
1. Pause the instrument with one of the Pause buttons on the Status screen.
2. Tap the appropriate Access retort button in the Paused dialog box.
3. Select to drain the retort or not in the Temporary access drain dialog box.
Follow the prompt to wait if draining.
4. When the Retrieve samples dialog box opens remove the baskets from the retort and add or
retrieve cassettes, as required.
5. Return the baskets to the retort and tap OK in the Retrieve samples dialog box.
6. If prompted, enter the new number of cassettes in the Number of cassettes dialog box.
Follow the prompt to wait if refilling the retort.
7. Tap Resume in the Paused dialog to resume the protocol.
CAUTION
Take care when adding fixed samples to a running protocol. The additional fixative will
contaminate the reagent used in the current step and this contamination will not be tracked by
the reagent management system.
CAUTION
The further a protocol has progressed before you add more samples the more compromised the
processing quality for those samples. We therefore recommend that you only add samples
during fixative steps or during the first dehydrant step.