Leica Biosystems PELORIS_PELORIS II - Manual (Page 135)

Reference
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 135
8.3 Station Configurations
Use the station configurations in this section for the protocols described in 8.2 Protocols.
If considering alternative station configurations remember to take into account how you will
configure your protocols, including the reagent selection method (see 4.1.2 Reagent Selection
Method). For example, some laboratories use explicitly graded alcohols with reagent types
identified as “Ethanol 70%”, “Ethanol 90%” etc. This sort of station configuration requires that
protocols use type (or station) selection, and of course the protocols must be configured to use the
alcohol grades in the correct order.
Xylene Configuration
This is the suggested station configuration when running xylene protocols (see 8.2.3 Xylene
Protocols). See note below for special conditions for initial bottle setup.
*All alcohol dehydrants after fixative should be labeled and named in the software “Ethanol”. However, if
setting up bottles on a new instrument or replacing all the ethanol bottles at once, the first and second bottles
should be filled with 70% and 90% ethanol respectively. As operation continues and you are prompted to
replace degraded bottles, replace with 100% ethanol. Other, initially higher-concentration bottles will have
degraded, so there will always be at least one bottle at a low concentration.
Station Reagent type Reagent group
Bottle 1 Formalin Fixative
Bottle 2 Formalin Fixative
Bottle 3 Ethanol* Dehydrant
Bottle 4 Ethanol* Dehydrant
Bottle 5 Ethanol Dehydrant
Bottle 6 Ethanol Dehydrant
Bottle 7 Ethanol Dehydrant
Bottle 8 Ethanol Dehydrant
Bottle 9 Ethanol Dehydrant
Bottle 10 Ethanol Dehydrant
Bottle 11 Xylene Clearer
Bottle 12 Xylene Clearer
Bottle 13 Xylene Clearer
Bottle 14 Xylene Clearer
Bottle 15 Cleaning xylene Cleaning solvent
Bottle 16 Cleaning ethanol Cleaning alcohol
Wax 1 Wax Wax
Wax 2 Wax Wax
Wax 3 Wax Wax
Wax 4 Wax Wax