Leica Biosystems PELORIS_PELORIS II - Manual (Page 136)

Reference
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 136
Xylene-free Configuration
This is the suggested station configuration when running xylene-free protocols (see 8.2.4 Xylene-
free Protocols). See note below for special conditions for initial bottle setup.
*All alcohol dehydrants after fixative should be labeled and named in the software “85% Ethanol”. However, if
setting up bottles on a new instrument or replacing all the ethanol bottles at once, the first two bottles should
be filled with 70% ethanol (but still be named “85% Ethanol”). As operation continues and you are prompted
to replace degraded bottles, replace with 85% ethanol. Other, initially higher-concentration bottles will have
degraded, so there will always be at least one bottle at a low concentration.
Station Reagent type
Bottle 1 Formalin
Bottle 2 Formalin
Bottle 3 85% Ethanol*
Bottle 4 85% Ethanol*
Bottle 5 85% Ethanol
Bottle 6 80/20 Ethanol/IPA
Bottle 7 80/20 Ethanol/IPA
Bottle 8 80/20 Ethanol/IPA
Bottle 9 IPA
Bottle 10 IPA
Bottle 11 IPA
Bottle 12 IPA
Bottle 13 Waxsol™
Bottle 14 Waxsol™
Bottle 15 Cleaning ethanol
Bottle 16 Cleaning ethanol
Wax 1 Wax
Wax 2 Wax
Wax 3 Wax
Wax 4 Wax