Leica Biosystems PELORIS_PELORIS II - Manual (Page 42)

Running Protocols
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 42
11. View the progress of the protocol in the Status screen.
You can pause processing to add additional cassettes, or abandon protocols completely (see
3.5 Pausing and Abandoning Protocols).
Finish Protocol Run
12. When the protocol is finished the Protocol complete dialog box opens and the alarm sounds;
tap OK to continue.
13. Tap Drain retort before accessing from the Drain/Access retort dialog box.
You can remove the baskets before draining the retort (tap Access retort now) – this option
is not recommended.
14. Wait for the Retort accessible dialog box to appear then open the retort and remove the
cassettes.
Tap OK to close the dialog box.
Run Cleaning Protocol
15. The Run cleaning protocol dialog box appears.
(i) Recommended: tap Clean now to load a cleaning protocol.
If you have more than one cleaning protocol use the Prev and Next buttons to select the
protocol you want to use.
(ii) Not recommended: tap Clean later to end the procedure and leave the retort full or with
residue in it.
If you select this option heating continues to keep the wax in the retort and valves molten.
See 3.2 Cleaning Protocols below for information about cleaning protocols.
16. Place the used cassette baskets into the retort when prompted with the Insert baskets dialog
box.
Remove all tissue as the cleaning protocol will damage any tissue left in the retort.
Tap Done in the dialog box when you have inserted the baskets and closed the retort.
17. Tap Start in the Scheduling dialog box to begin the run.
If required change the protocol’s end time before starting.
18. View the progress of the protocol in the Status screen.
19. When the protocol is finished the Protocol complete dialog box opens and the alarm sounds;
tap OK to continue.
20. When the Remove baskets dialog box appears, open the retort lid and remove the baskets.
Tap OK to continue.
21. Tap Unload protocol in the Status screen so the retort is available for the next protocol.
WARNING
Do not open a retort while a protocol is running as the retort could be pressurized and may
contain hot reagent and fumes. Always follow the retort access instructions detailed in 3.5
Pausing and Abandoning Protocols if you need to access a retort during processing.