Leica Biosystems PELORIS_PELORIS II - Manual (Page 57)

Running Protocols
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 57
3.6.3 Unavailable Reagents
Once a protocol starts, group and type select protocols may reassign stations to recover from errors
caused by unavailable reagents (for example when a bottle is removed or if a station contains
insufficient reagent). This reassignment may use reagents assigned to the other protocol.
Station-selection protocols will fail if an assigned reagent becomes unavailable. Type-selection
protocols will fail if there is only one station with an assigned type and it becomes unavailable.
Some common causes of station unavailability, and ways to avoid them, are described below.

The station contains insufficient reagent.
Prior to each run, check the reagent level in each station is sufficient for the current fill level
(see Instrument settings screen for the reagent fill level).

A bottle scheduled for use is removed from the reagent cabinet.
For safety reasons you should not remove any bottle whilst a protocol is running. However if
you choose to do so, you must make sure the bottle you intend to remove is not scheduled for
use in either retort.

A wax station is not molten at the time it is required.
Make sure there is adequate time for the wax to melt and that the correct wax station state is
set (see 5.3.2 Setting Reagent Station Properties).