3 managing reagent stations, 1 reagent stations screen, Managing reagent stations reagent stations screen – Leica Biosystems PELORIS_PELORIS II User Manual

Page 84: 3 managing reagent, Stations, See 5.3 managing reagent stations and

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Reagent Setup

Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011

84

5.3 Managing Reagent Stations

The Peloris processor has 20 stations – 16 reagent bottles and four wax chambers.

You must set the stations in the software to the reagent types loaded on the instrument. Once this

is done the system tracks each station’s use history (the numbers of runs and cassettes processed

and number of days loaded on the instrument), current concentration, and, for wax chambers, the

current temperature. See Stations in 5.1.1 Reagent Groups, Types and Stations for further details.

Supervisors can set the reagent type for each station and change concentration values if they know

that actual concentrations are different from those in the system. Both supervisors and operators

can change station states if required. The use history details can only be viewed.

It is essential for safe operation of the Peloris that there are bottles loaded into all bays in the

reagent cabinet. If you do not wish to use a particular station, set its state to Dry and (for non wax

stations) insert an empty bottle into the station’s reagent cabinet location.

The choice of which reagent types to load on the instrument, and how many bottles of each, is an

important question that you must decide along with consideration of the protocols you run. See 8.3

Station Configurations for configurations suitable for the default protocols.

5.3.1 Reagent Stations Screen

Use the Reagent stations screen (Reagents menu, Stations) to set up and manage reagent

stations. There are two views of the screen – one for the 16 reagent bottles and the other for the

four wax chambers. Change views with the Reagent bottles and Wax chambers buttons.

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