Leica Biosystems PELORIS_PELORIS II - Manual (Page 133)

Reference
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 133
Xylene-free 8 Hour

Pre-defined protocol “Factory 8 hr Xylene Free“

Carryover setting in pre-defined protocol: 26
Xylene-free 12 Hour

Pre-defined protocol “Factory 12 hr Xylene Free“

Carryover setting in pre-defined protocol: 26
Step Reagent type Time (min) Temp (°C) P/V Stirrer
Drip time
(s)
1 Formalin 30 55 Ambient Medium 10
2 85% Ethanol 20 55 Ambient Medium 10
3 85% Ethanol 30 55 Ambient Medium 10
4 80/20 Ethanol/IPA 30 55 Ambient Medium 10
5 80/20 Ethanol/IPA 60 55 Ambient Medium 10
6 IPA 20 55 Ambient Medium 10
7 IPA 40 55 Ambient Medium 10
8 IPA 80 55 Ambient Medium 10
9 Wax 60 85 Vacuum Medium 10
10 Wax 50 85 Vacuum Medium 10
11 Wax 40 65 Vacuum Medium 10
Processing time 8:04:00
Step Reagent type Time (min) Temp (°C) P/V Stirrer
Drip time
(s)
1 Formalin 68 55 Ambient Medium 10
2 85% Ethanol 30 55 Ambient Medium 10
3 85% Ethanol 40 55 Ambient Medium 10
4 80/20 Ethanol/IPA 50 55 Ambient Medium 10
5 80/20 Ethanol/IPA 90 55 Ambient Medium 10
6 IPA 30 55 Ambient Medium 10
7 IPA 60 55 Ambient Medium 10
8 IPA 120 55 Ambient Medium 10
9 Wax 80 85 Vacuum Medium 10
10 Wax 70 85 Vacuum Medium 10
11 Wax 60 65 Vacuum Medium 10
Processing time 12:02:00