Leica Biosystems PELORIS_PELORIS II - Manual (Page 97)

Reagent Setup
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 97
Fill
7. Fill the wax chamber with molten or solid wax.
8. Open the Reagent stations screen, wax chambers view (Reagents menu, Stations, then
tap the Wax chambers button).
The row for the drained wax chamber shows the default concentration and all use history
values set to zero. The station state is Dry.
The reagent type is unchanged from the wax that you drained unless you changed the type
assigned to the station after the drain (see Assigning New Reagents to Stations)
9. Tap the State cell for the chamber:

If using molten wax, set the state to Full.

If using solid wax, set the state to Not molten to begin the rapid wax heating process.
While in the Not molten state the wax bath chamber temperature is higher than normal
to ensure the wax melts quickly.
You may need to add extra wax as it melts.
The station’s state automatically changes to Full when the wax is ready to use.
5.4.6 Filling and Draining Retorts
The Remote fill/drain screen allows you to fill and drain the retorts to assist recovery from
incomplete remote fill/drain operations. The retort fill and drain functions operate with a series of
rules designed to avoid reagent contamination, reagent overflow spills and reagent overheating.
You can override some rules but this will often result in reduced reagent concentration.
You can also fill and drain retorts using the Manual operations screen (see 6.1.1 Manual
Operations).
The rules for manually filling and draining retorts are as follows.

The retort must be clean or empty before you commence a retort fill operation.

If the retort is empty the selected station must have compatible reagent (see 8.5 Reagent
Compatibility Tables).

You cannot fill a retort with a reagent that has a temperature threshold below the set retort
temperature.

When draining a retort, the reagent must return to its original station.

When draining a retort, the station must have sufficient capacity for the retort contents.
To avoid fluid spills, ensure adequate station capacity before overriding insufficient capacity errors.
To fill or drain a retort select the retort and the reagent station to fill from or drain to, on the
Remote fill/drain screen. Tap the Fill retort or Drain retort button. To stop the fill at any
stage, tap the Abandon button.
WARNING
Never fill a reagent station when its contents are in a retort. This may cause fluid spills.