Leica Biosystems PELORIS_PELORIS II - Manual (Page 147)

Troubleshooting
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 147
9.2.7 Staining Artifact
Is there cellular
detail?
Under/Over processed
Change or
replace solutions in reagent bottles
Ensure protocol step times are
adequate
Is there formalin
pigment?
pH of formalin is too
high
Fix tissue in neutral
buffered formalin,
and change
regularly
Is there coarse or
fine cracking
?
Over processed
Change or replace
solutions in reagent
bottles
Ensure protocol step times
are adequate
Is there zonal
fixation?
Under fixed
Make sure tissue is placed in
an adequate amount of formalin for an adequate
amount of time. As soon as possible after removal from
patient
Ensure tissue is not too big for
cassette
Does the tissue have blue hue or smudge?
Water present in
tissue
Change or replace solutions in
reagent bottles
Increase step time in
dehydration
See sections
9.2.1, 9.2.2, 9.2.3
No
Yes
Yes
Yes
See sections
9.2.1, 9.2.2, 9.2.3
Inadequate
fixation