Priming and purging the systems, Priming and purging the systems on – Bio-Rad ChromLab™ Software User Manual
Page 120
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| Preparing the Instrument
118
| NGC Chromatography Systems and ChromLab Software
Priming and Purging the Systems
This section explains how to prime your system before running the pumps, and
how to purge your system of air bubbles or rapidly exchanging buffer
solutions.
Note:
This section assumes that you primed the pump head wash system
when you installed the system. See
Plumbing All NGC Systems on page
for
more information.
Priming and purging tasks are specific to the NGC system configuration. Table
lists the tasks that are associated with each system configuration. The tasks are
explained in detail in the sections that follow.
Table 13.
Priming and purging tasks for each NGC system configuration
NGC Quest
NGC Scout
NGC Discover
Prime and purge the
inlet port on system
pump A.
Prime and purge Q1–Q4
on the buffer blending
valve.
Tip:
The inlet ports
on system pumps A
and B are primed
and purged when
the buffer blending
valve is primed.
Prime and purge Q1–Q4 on
the buffer blending valve.
Tip:
The inlet ports on
system pumps A and B
are primed and purged
when the buffer
blending valve is
primed.
Prime and purge the
inlet port on system
pump B.
Prime and purge the buffer
inlet valves A and B.
Prime and purge the sample
pump.