Bio-Rad ChromLab™ Software - Manual (Page 136)

3 | Preparing the Instrument
134 | NGC Chromatography Systems and ChromLab Software
2. To purge an individual line of air bubbles and fill it with buffer:
a. Select the port to open.
b. (Optional) Change the default flow rate.
The default flow rate is set to 20 ml/min.
c. Click Start.
d. Repeat steps 2a–c for any other port to purge.
3. To purge all lines of air bubbles and fill them with buffer:
a. Select All.
b. (Optional) Change the default flow rate.
The default flow rate is set to 20 ml/min.
c. Click Start.
The system pumps and the buffer blending valve start operating at the
specified flow rate, exchanging the solution in the inlet lines and expelling any
trapped bubbles, cycling sequentially through the ports Q1 to Q4. After the
specified duration, the pumps stop momentarily while the blending valve
switches to port Q3 in order to flush the lines with water before closing the
valve. No change is allowed during this process for the specified duration.
Note: You can stop the cycle before it completes by clicking Stop. In this
case, ensure that all buffer lines are filled with the appropriate buffers
before continuing.